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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Curr Protoc Cytom. 2018 Dec 17;88(1):e54. doi: 10.1002/cpcy.54

Fig. 5. Representative data from cytometry measurements.

Fig. 5.

(Top) Logarithmic dot-plot fluorescent measurements of ~10 000 cells in P3 that have been transfected with plasmids expressing either the sensor or cpVenus, as indicated. Each column represents data determined from the indicated subcellular compartment. x-axis, fluorescence following 405 nm excitation; y-axis, fluorescence following 488 nm excitation. (Bottom) Histograms showing the distribution of the same P3 population as a function of the 488/405 nm ratio per cell. Data was collected on a BD LSRII flow cytometer and analyzed on FlowJo V10 software.