Table 2. Measurements needed for flow cytometry analysis.

The voltage guidelines are provided here only as aides for setting up a template. They must be adjusted by the user such that all measurements are on-scale for the instrument’s specific lasers and set-up. Different instruments may have different voltage settings or may not have voltage controls.

Name	Scale	Approximate voltage guidelines for HeLa cells
SSC-A	Linear	~275
FSC-A	Linear	~220
SSC-H	Linear	No Recommendation
SSC-W	Linear	No Recommendation
Filter Set: Ex. 488 nm, Em. 530 ± 15 nm	Log	~275
Filter Set: Ex. 405 nm, Em. 525 ± 25 nm	Log	~250