(A) The mRNA expression of α-SMA, SM22α, calponin and osteopontin in VSMCs transfected with siR-MRAK048635_P1 compared with Scr siRNA. The data were representative of three independent experiments with five rats per group. (B,C) The protein expression of α-SMA, SM22α, calponin, and osteopontin in VSMCs transfected with siR-MRAK048635_P1 compared with Scr siRNA. (D,E) Immunofluorescence showed the expression of α-SMA and calponin in VSMCs transfected with siR-MRAK048635_P1, and quantitated using ImagePro Plus software. *P<0.05, **P<0.01, compared with Scr siRNA.