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. 2019 Mar 12;9:55. doi: 10.3389/fcimb.2019.00055

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Copyright © 2019 Wholey, Abu-Khdeir, Yu, Siddiqui, Esimai and Dawid.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functional analysis of the BIR region of P164 strain after moving into a 19A strain background. (A) Schematic representation of BIR_P164. Blue arrows indicate predicted pneumocins, and green arrows indicate predicated immunity proteins. Black arrows indicate ORF encoding proteins of unclear significance. Curved arrows represent predicted transcriptional start sites based on the presence of a predicted BlpR binding site. (B) Agar overlay assays showing inhibitory and immune phenotypes. The properties of strains spiked into the plate are shown below the photo, overlay strains designations are shown above the photo.