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. 2019 Mar 12;9:51. doi: 10.3389/fcimb.2019.00051

Figure 6.

Figure 6

Analysis of the fungal mycelia reisolated from plants inoculated with the virus-free (V−) and the virus-infected (V+) strain of Fusarium oxysporum f. sp. dianthi isolate 77. (A) Amplification products obtained in multiplex-PCR reactions using specific primers for the different races-groups described in F. oxysporum f. sp. dianthi (Fod) and DNA from the fungal colonies recovered from root, crown, or stem of carnation plants inoculated with the virus-free strain V− or the virus-infected strain V+ of isolate Fod 77. Specific primers used and conditions of the multiplex-PCR were as described in Gómez-Lama Cabanás et al. (2012). M, molecular weight marker XIV (Roche Diagnostics). R2I, R1t, and R2II, representative of the respective race-group described in F. oxysporum f. sp. dianthi. (B) Extracts of dsRNA obtained by cellulose column chromatography using mycelia recovered from a carnation plant inoculated with the virus-free (V−) or the virus-infected (V+) strain of isolate Fod 77. M, 1Kb DNA Ladder (Nippon Genetics).