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. 2019 Mar 12;9:51. doi: 10.3389/fcimb.2019.00051

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Copyright © 2019 Torres-Trenas, Prieto, Cañizares, García-Pedrajas and Pérez-Artés.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Isolates of Fusarium oxysporum f. sp. dianthi infected with mycovirus FodV1. (A) dsRNA-enriched extracts obtained by cellulose column chromatography from isolates Fod 116 (the originally infected isolate), Fod 183, Fod 185, and Fod 211. (B) Specific amplicons obtained after RT-PCR of the above dsRNA extracts using specific primers for the RdRp sequence of FodV1. M, molecular weight marker II (Roche Diagnostics) (see also Supplementary Image to Figure 7).