Table 1.
Fusarium oxysporum f. sp. dianthi isolates analyzed for the presence of FodV1 viral infection.
| Isolate (s)a | Geographic originb | Source | Year | Race group assignation by PCR patternc |
|---|---|---|---|---|
| Fod 108, 110, 111, 120, 121 | Chipiona(Ca) | Plant | 2008 | R1t |
| Fod 112, 113, 114, 117 | Chipiona(Ca) | Plant | 2008 | R2I |
| Fod 118, 119 | Chipiona(Ca) | Plant | 2008 | R2II |
| Fod 115 | Chipiona(Ca) | Plant | 2008 | – |
| Fod 124, 127, 132, 134, 136, 138 | Chipiona(Ca) | Plant | 2009 | R2I |
| Fod 122, 128, 130, 140, 142 | Chipiona(Ca) | Plant | 2009 | R1t |
| Fod 183, 185, 187 | Chipiona(Ca) | Plant | 2010 | R2I |
| Fod 181, 189, 191 | Chipiona(Ca) | Plant | 2010 | R1t |
| Fod 179, 195, | Chipiona(Ca) | Soil | 2010 | R2II |
| Fod 197 | Chipiona(Ca) | Soil | 2010 | R1t |
| Fod 144, 146, 148, 150, 158, 160, 162, 164 | La Colonia(Se) | Plant | 2010 | R2I |
| Fod 152, 154 | Lebrija(Se) | Plant | 2010 | R1t |
| Fod 156 | Lebrija(Se) | Plant | 2010 | R2II |
| Fod 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 245, 247, 249, 251, 253 | Chipiona(Ca) | Plant | 2011 | R2I |
| Fod 200, 201, 203, 205 | Chipiona(Ca) | Plant | 2011 | R1t |
| Fod 207 | Chipiona(Ca) | Soil | 2011 | R2I |
| Fod 256, 258, 259, 260, 261, 264, 265, 267, 269, 272, 275, 281 | Chipiona(Ca) | Soil | 2011 | R2II |
| Fod 270, 280 | Chipiona(Ca) | Soil | 2011 | R1t |
| Fod 211 | Lebrija(Se) | Plant | 2011 | R2I |
| Fod 207, 210, 213, 215, 217, 219, 221 | Lebrija(Se) | Plant | 2011 | R2I |
| Fod 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 341.1, 357, 358, 359, 360, 361, 364, 368, 373.2, 433, 434, 435, 453 | Chipiona(Ca) | Plant | 2012 | R2I |
| Fod 365, 452 | Chipiona(Ca) | Plant | 2012 | R2II |
| Fod 341.2, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 363, 366, 367, 371, 372, 373.1, 471 | Chipiona(Ca) | Plant | 2012 | R1t |
| Fod 337, 338, 339, 340, 369, 370, 375, 437, 438, 439, 442, 443, 444, 445, 446, 451, 469 | Chipiona(Ca) | Soil | 2012 | R2II |
| Fod 362, 376, 440, 441, 447, 448, 450, 454, 459, 463, 476 | Chipiona(Ca) | Soil | 2012 | R2II |
| Fod 352, 353, 354, 355, 356, 374, 449, 455, 456, 457, 458, 460, 461, 462, 464, 465, 466, 467, 468, 470, 472, 473, 474, 475, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487 | Chipiona(Ca) | Soil | 2012 | R1t |
| Fod 383, 384, 385, 386, 387, 388 | Lebrija(Se) | Plant | 2012 | R2I |
| Fod 377, 378, 379, 380, 381, 382, 391, 392, 393, 394 | Lebrija(Se) | Plant | 2012 | R1t |
| Fod 400, 402 | Lebrija(Se) | Soil | 2012 | R2I |
| Fod 389, 390, 395, 403, 404, 405 | Lebrija(Se) | Soil | 2012 | R2II |
| Fod 396, 397, 398, 399, 401 | Lebrija(Se) | Soil | 2012 | R1t |
a
Isolates had been previously characterized to race-group by specific-PCR amplifications, as described in Gómez-Lama Cabanás et al. (2012).
dsRNA from each isolate was purified by cellulose column chromatography and analyzed by RT-PCR using specific primers FodV1f/FodV1r directed to the RdRp sequence of mycovirus FodV1 (Lemus-Minor et al., 2018).
b
Ca, Cádiz province; Se, Sevilla province.
c
Race-group assignation by molecular markers. R1t, race 1molecular group type; R2I, race 2 molecular group I; R2II, race 2 molecular group II.
(–) Unknown data.