Table 2.
Semi-quantification of sortilin immunoreactivity in major neuroanatomical regions and neuronal populations in the adult human brain.
| Structure and neuronal types | Immunolabeling intensity | Specific note |
|---|---|---|
| Olfactory bulb | ||
| Periglomerular neurons | (-) | |
| Mitral and tufted cells | (-) | |
| Granule cells | (-) | Subependymal cells (+) |
| Cerebral neocortex | ||
| Layer I | (-/+) | Large pyramidal neurons in layers III and V (++++); interneurons (+/++) |
| Layer II/III | (+++) | |
| Layer IV | (+/++) | |
| Layer V | (+++) | |
| Layer VI | (+++) | |
| White matter | (-) | |
| Amygdala and basal forebrain | ||
| Central nucleus | (+++) | |
| Basolateral nucleus | (+++) | |
| Lateral nucleus | (++) | |
| Basomedial nucleus | (++/+++) | |
| Basal nucleus of Meynert | (+++) | Sortilin and ChAT colocalization observed |
| Hippocampal formation | ||
| Entorhinal cortex | (+++) | |
| Prosubiculum | (+++) | |
| Subiculum | (+++) | |
| Presubiculum | (+++) | |
| Parasubiculum | (+++) | |
| CA1-CA3 (pyramidal neurons) | (+++) | Thorny excrescences on CA3 and mossy cells (+++); interneurons (+/++) |
| Hilus (mossy cells) | (+++) | |
| Granule cells | (++++) | |
| Basal ganglia | ||
| Caudate | (++) | Large spiny ChAT neurons (++++); small spiny neurons (++) |
| Putamen | (+) | |
| Globus pallidus | (++) | |
| Claustrum | (+++) | |
| Diencephalon | ||
| Thalamus | ||
| anterior nuclear group | (++) | |
| medial nuclear group | (++) | |
| posterior nuclear group | (++) | |
| lateral geniculate nucleus | (++) | |
| medial geniculate nucleus | (++) | |
| Hypothalamus | (++) | |
| Mesenphalon (midbrain) | ||
| Substantia nigra pars compacta | (+++) | Sortilin/TH colocalization observed |
| Substantia nigra pars reticulata | (-/+) | |
| Ventral tegmentum area | (++) | |
| Red nucleus | (++) | |
| Periaqueductal gray | (++) | |
| Oculomotor nucleus (CN3) | (++) | |
| Trochlear nucleus (CN4) | (++) | |
| Superior colliculus | (++/+++) | Large multipolar cells in the deep layers (+++) |
| Inferior colliculus | (++/+++) | |
| Pons | ||
| Locus coeruleus | (++++) | Sortilin/TH colocalization observed |
| Motor trigeminal nucleus (CN5) | (+++) | |
| Sensory trigeminal nucleus (CN5) | (++) | |
| Median raphe nucleus | (+++) | |
| Pontine nucleus | (++) | |
| Superior olivary nuclei | (++) | |
| Medulla oblongata | ||
| Vestibular nuclei (CN8) | (+++) | |
| Cochlear nuclei (CN8) | (+++) | |
| Inferior olivary nuclei | (++) | |
| Nucleus of the solitary tract | (+++) | |
| Spinal trigeminal nucleus (CN5) | (+++) | |
| Abducent nucleus (CN6) | (+++) | |
| Facial nucleus (CN7) | (+++) | |
| Dorsal motor nucleus vagus (CNX) | (+++) | |
| Hypoglossal nucleus (CN12) | (+++) | |
| Cerebellum | ||
| Molecular layer (basket cells) | (+/++) | |
| Purkinje cell layer (Purkinje cells) | (-/+) | Extracellular granular profiles (++) |
| Granule cell layer (granule cells) | (-/+) | |
| White matter | (-) |
(-), background level as seen in the white matter; (+), weak labeling with cell outline visible; (++), moderate labeling with cell body and dendritic processes well visualized; (+++), strong labeling with somata and dendritic processes distinctly visualized; (++++), strongest labeling with somata and dendrites heavily stained; CN, cranial nerve nucleus; ChAT, choline acetyltransferase; TH, tyrosine hydroxylase.