Skip to main content
. 2019 Mar 12;10:205. doi: 10.3389/fneur.2019.00205

Figure 2.

Figure 2

sICAM-5 does not affect Th17—APC interactions. (A) FACS staining for LFA-1 of naïve T-cells, Th17 cells, and Th17 cells after 24 h of stimulation with anti-CD3/CD28 (n = 3 for each condition). (B) Representative depiction of CFSE stainings of naïve 2d2 T cells cocultured with APCs and sICAM-5 (10 μg/ml) or IgG (10 μg/ml) for 72 h. (C) FACS staining of Th17 cells stimulated for 4 and 24 h with anti-CD3/CD28 and with or without ICAM-5 treatment (10 μg/ml). (D,E) Naïve T cells were differentiated into Th17 cells in the presence of or without sICAM-5 treatment (10 μg/ml) and stained for extracellular (D) and intracellular (E) markers. Data shown are mean ± SEM. (A) One-way ANOVA followed by Tukey's multiple comparison test, n.s. no significance; (C–E) Student's t-test.