Figure 6.

LPS priming increases spontaneous migration of neutrophils in the absence of chemoattractant. Migration counts of neutrophils (dHL-60s) that migrated in the absence of chemoattractant (n = 3 separate experiments). (A) High and super-low dose LPS treatment significantly increases the percentage of cells that spontaneously migrate into the migration channels of the microfluidic competitive chemotaxis-chip in the absence of chemoattractant. Blue depicts the unstimulated neutrophils, green the neutrophils stimulated with a super-low dose of LPS, and red the neutrophils stimulated with a high dose of LPS. (B) Percentages of migrating cells in the absence of chemoattractant in all three treatment groups. LPS stimulation significantly increases spontaneous dHL-60 cell migration in the absence of chemoattractant. Data expressed as means and standard deviations. *p < 0.05.