Fig. 1.

Adipose stem cells (ASCs) Migrate toward Conditioned Media (CM) from Chemo-Residual TNBC Cells. A CM was prepared by growing chemo-residual SUM159 TNBC cells in serum-free media for 48 h. The ability of primary human ASCs (Zenbio) to migrate toward this CM (Chemo-residual CM), FBS (+ Control) or serum-free media (− Control) was evaluated in a 15 h transwell assay. Total number of migrated cells from 5 representative fields (100 × magnification) was determined for each well, and mean cell number from triplicate wells (± SD) was calculated. Significance was determined by two tailed t test (****p < 0.0001). Similar results were obtained in at least three independent trials. B Representative fields (100 ×) showing migration of ASCs towards chemo-residual TNBC CM in a 15 h transwell assay. C CXCR4 expression was assessed by incubating ASCs with CXCR4 antibody (blue line) or control IgG (red line), followed by FITC-conjugated secondary antibody. CXCR4 cell surface expression was determined by flow cytometry. D RNA was isolated from untreated and chemo-residual SUM159 cells and subjected to SDF-1 alpha and GAPDH real-time PCR. SDF-1 alpha gene expression relative to GAPDH is presented for both cell lines