FIGURE 7.

The nucleosome-guided cfDNA fragmentation pattern facilitates the prediction of amplicon coverage uniformity across samples. The ANOVA test demonstrates dependency between spanning fragment counts and coverage uniformity as well as read depth overage shape and coverage uniformity (C,D), though no similar relation to the mean amplicon coverage across the samples was observed (A,B). The SVM classification model utilizing the GC content as a single feature, or in combination with the cfDNA fragmentation pattern defining features, demonstrates the significance of the latter to predict amplicon coverage uniformity across samples (F), but not of the mean sequencing coverage across samples (E).