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. 2019 Mar 12;10:252. doi: 10.3389/fphys.2019.00252

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Copyright © 2019 Zhu, Umehara, Okazaki, Goto, Fujita, Hoque, Kawai, Zeng and Shimada.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of D-chloramphenicol (CRP) on boar sperm at 3 h incubation with different doses (0, 200, 400, 600, 800 ng/mL). (A–F) Dose-dependent changes in the sperm (A) total motility, (B) non-progressive motility, (C) progressive motility, (D) straight-line velocity, (E) mitochondrial activity, and (F) ATP level. (G) Western blotting image showing the expression of the MT-ND1, MT-ND6, NDUFA7, and α-tubulin. (H–J) Quantitative expression of the MT-ND1, MT-ND6, and NDUFA7 over α-tubulin (control) generated from western blotting. (K) Membrane integrity measured in sperm. Values are means ± SEM of three replicates. Columns with different lowercase letters differ significantly (p < 0.05).