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. 2019 Mar 18;9:4782. doi: 10.1038/s41598-019-41108-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot of subcellular fractionation of the mitochondrial fraction (M) into OMM and mitoplast (IMM and matrix) with 1.2% digitonin. The M/S-isolated mitochondrial fraction was permeabilised with digitonin over time (0 min, 15 min, 20 min and 30 min) and separated into an OMM and mitoplast (IMM and matrix) fraction with differential centrifugation prior to western blotting with antibodies for ATM (350 kDa), VDAC (32 kDa) and ANT (34 kDa). The images were cropped and the uncropped blots as well as total protein membrane images are presented in Supplementary Material Fig. S2. The molecular weight marker (HiMark pre-stained protein standard, ThermoFischer Scientific) was loaded in lane 1 (mm) and sizes are indicated on the right-hand side of each panel. Samples derived from the same experiment were blotted for VDAC and ANT (2 separate membranes due to similar size), and samples derived from the same experimental procedure, but on a different day for ATM, are shown here.