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. 2019 Mar 18;9:4674. doi: 10.1038/s41598-019-40718-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Principle of guided STED for malaria samples. In conventional STED super-resolution microscopy (top row), the infected red blood cell is scanned with the STED laser (red arrow) at full power in every pixel leading to disintegration of hemozoin particles (yellow) and sample destruction. Guided STED (bottom row) can test for the presence of hemozoin at every pixel using light reflected by hemozoin particles (probing). To avoid sample damage during the probing step, the depletion laser is running at a very low power (pink). Only where the reflected light signal is below the threshold T_S, the depletion laser is switched back to high power (red arrow) for super-resolution imaging.