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. 2019 Mar 18;9:4674. doi: 10.1038/s41598-019-40718-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sample damage in conventional or guided STED. Representative confocal images of infected red blood cells stained for the parasitophorous vacuole (EXP2, cyan) and Maurer’s clefts (SBP1, magenta) and hemozoin visualisation (yellow) using reflected light from the excitation laser. Images were recorded after single rounds of STED imaging with increasing powers (0.49, 24, 47, 71, 91, 214 or 474 mW) of the STED laser to compare damage inflicted in conventional STED mode, conventional STED mode with illumination breaks and guided STED mode. Examples of three major stages of sample damage: A – altered hemozoin structure, B – damages of fluorophores in the proximity of hemozoin, C – hemozoin disintegration. Scale bar 2 μm.