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. 2019 Mar 18;9:4837. doi: 10.1038/s41598-019-41258-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Prenatal allergic and postnatal immune challenge models. Ovalbumin (OVA) sensitized or control females were bred and challenged intranasally with OVA or vehicle on gestational day (GD) 15, and pups assessed neonatally for brain-resident immune cell numbers or grown to adulthood for sociosexual behavior testing and neuroanatomical assessment. Neonatal offspring from naive dams were challenged on the day of birth with an intraperitoneal injection of lipopolysaccharide (LPS), and the POA assessed on PN4 for spinophilin protein content.