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. 2019 Mar 18;9:4696. doi: 10.1038/s41598-019-41140-1

Figure 6.

Figure 6

Cordycepin treatment reduces monosodium iodoacetate (MIA)-induced subchondral bone changes. OA was induced by injecting MIA (1 mg/50 µl) in the left knee joints on day 0. At day 14, cordycepin (Cordy; 8 mg/kg, orally, every other day) or vehicle (Veh) was administered for a period of 2 weeks until day 28. Saline (50 µl) injected rats were used as controls. Cordycepin treatment reduced MIA-induced increase in number of channels crossing the osteochondral junction (OCJ) (A,B), tartrate-resistant acid phosphatase (TRAP) positive osteoclasts (A,C) as well as nestin (A,D) and VEGF (A,E) expression in the subchondral bone. Vehicle-treated saline-injected control rats showed fewer number of channels crossing the OCJ and TRAP positive osteoclasts as well as nestin and VEGF expression in the subchondral bone compared with MIA rats. Coronal sections of rat joints were stained for Safranin-O fast green showing histological changes in the cartilage and subchondral bone (OCJ), TRAP positive osteoclasts), nestin and VEGF immunoreactivity. Scale bar = 100 µm. Data are presented graphically as mean ± SEM from n = 10 rats/group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus vehicle-treated saline-injected controls. +p < 0.05, ++p < 0.01, +++p < 0.001, ++++p < 0.0001 versus vehicle-treated MIA-injected OA rats at day 28. #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001 versus MIA-injected OA rats at day 14.