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. 2019 Mar 18;9:4684. doi: 10.1038/s41598-019-41288-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Promoter activity analysis of ToElovl4a gene. A. The structure and transcriptional activity of the ToElovl4a promoters. Five recombinant plasmids, denoted as Elovl4a-1 (−148 to +56), Elovl4a-2 (−500 to +56), Elovl4a-3 (−1001 to +56), Elovl4a-4 (−148 to +155) and Elovl4a-5 (−148 to +258), were constructed and transfected with transcription factor PPARαb into HEK 293 T cells. B. Dual luciferase activity driven by the ToElovl4a-5 core promoter upon the transfection of pcDNA3.1-PPAR-α and pcDNA3.1 in HEK 293 T cells. All values are presented as the means ± SD (n = 3). Asterisks indicate that the values are memorably different from the individual controls (*p < 0.05 and **p < 0.01). Bars on the same group with different letters are statistically significant from one another.