Figure 2.

Regional distribution of MUC5B and MUC5AC mRNA and protein localization in normal/healthy human airways. Serial sections from five different regions of airways from one normal/healthy lung were stained by hematoxylin and eosin and Alcian Blue and periodic acid–Schiff, and probed for MUC5B and MUC5AC by RNA in situ hybridization and immunohistochemistry. Scale bars = 40 μm. For details on the methods of image acquisition, see Quantitation of mucous glycoproteins, MUC5AC, MUC5B, and CCSP in AB-PAS, immunohistochemistry and RNA ISH in the online supplement. AB-PAS = Alcian Blue and periodic acid–Schiff; H&E = hematoxylin and eosin; IHC = immunohistochemistry; ISH = in situ hybridization.