Fig. 2. Knockdown of Akirin2 suppresses the proliferation, migration and invasion of cholangiocarcinoma (CCA) cells in vitro.

a Western blot analysis was employed to examine the efficiency of Akirin2 knockdown. b Proliferation curves were determined in Akirin2 stable knockdown CCLP1 and RBE cells by cell counting kit-8 (CCK-8) assays. c Colony-forming abilities were measured in Akirin2 stable knockdown CCLP1 and RBE cells by clonogenic assays. d Wound-healing assay was performed to measure the migration ability of various cells as indicated. e, f Transwell assays were used to detect the migration and invasive capacities in Akirin2 stable knockdown CCLP1 and RBE cells. Magnification, × 40 (d), × 200 (e, f). Scale bar, 500 μm (d), 100 μm (e, f). **P < 0.001; ***P < 0.001. Data are shown as mean ± SD of at least three independent experiments