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. 2019 Mar 12;10:445. doi: 10.3389/fimmu.2019.00445

Figure 3.

Figure 3

RELM-β expression in exfoliated cells reflects the intensity and type of infection. BALB/c mice were infected orally with either a low dose (20 L3) or a high dose (200 L3) H. polygyrus larvae. (A) Worm burden assessed at 14 dpi. (B) RELM-β relative gene expression during early H. polygyrus infection until day 8 dpi after low dose (20 L3) and high dose (200 L3) infection. (C) BALB/c mice were infected orally with 200 L3 H. polygyrus, treated with pyrantel pamoate for worm clearance and 28 days later reinfected with 200 L3. RELM-β gene expression shown during primary and secondary infection. (D) Linear regression comparing RELM-β gene expression at day 8 in exfoliated cells with low worm burden. (A) is representative from two independent experiments with n = 3–5 animals and shows the mean ± SD. (B) is pooled from two independent experiments with n = 4–6 animals, data shown as mean ± SEM. All relative gene expression analysis is compared to β-actin using 10ng cDNA. (C) is representative of two independent experiments, n = 4–5. (D) is pooled from two independent experiments, n = 10. Statistical analysis using (A) Mann Whitney U Test, (B) multiple t tests and corrected for multiple comparisons using the Holm-Sidak method, (C) was performed using the Kruskal-Wallis with Dunn's multiple comparison test. All relative gene expression analysis is compared to β-actin. Significance was measured as *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001.