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. 2019 Mar 12;10:478. doi: 10.3389/fmicb.2019.00478

FIGURE 3.

FIGURE 3

Bi-directional Blast reveals high number of fragmented CDS in S. saccharolyticus. S. epidermidis ATCC12228 was used as reference genome and the number of orthologs in other closely related staphylococcal species are given, when applying different protein coverage cutoffs (25, 50, 75, and 85%). For instance, S. saccharolyticus strain 05B0362 shares 1,912 CDS (with the reference genome) with a minimum protein sequence coverage of 25%, but only 1,527 CDS with a minimum coverage of 85%. In contrast, in S. capitis the number of orthologous CDS remained high even at a coverage cutoff of 85%. This indicates gene decay in S. saccharolyticus, leading to fragmented CDS. The bi-directional Blast was carried out with the program Proteinortho.