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. 2019 Mar 12;10:478. doi: 10.3389/fmicb.2019.00478

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Copyright © 2019 Brüggemann, Poehlein, Brzuszkiewicz, Scavenius, Enghild, Al-Zeer, Brinkmann, Jensen and Söderquist.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hyaluronidase activity of S. saccharolyticus. Detection of hyaluronate lyase activity with a hyaluronic acid plate assay. Colonies of S. saccharolyticus strains were point-inoculated onto hyaluronic acid-containing plates and incubated for 48 h under anaerobic conditions. Plates were flushed with 2N acetic acid for 15 min for the detection of hyaluronic acid degradation. Numbers (in brackets the respective S. saccharolyticus subclade): 1, positive control (hyaluronidase from Streptococcus pyogenes); 2, 05B0362 (1); 3, 12B0021 (1); 4, 13T0028 (1); 5, DVP2-17-2406 (2); 6, DVP3-16-6167 (1); 7, DVP4-17-2404 (2); 8, DVP5-16-4677 (2); 9, S. epidermidis FSI; 10, S. epidermidis 14.1.R1; 11, BHCY medium (negative control). The pictures are representative of three independent experiments.