Table 2.

CRISPR applications in cancer research

Application	Targets	sgRNA design	Vehicle for delivery	Features	Advantages	Disadvantages
Generate cancer model	HSPCs; healthy human organoids	Targeting the model type-related suppressors oncogenes	Pooled lentivirus	Disrupt suppressors or edit oncogenes	Rapid, efficient, and inexpensive	Special delivery techniques; tissue limited
Synergistic gene study	Cells	Targeting optional drug target from database	Lenti-double sgRNA library	Together with deep sequencing	Effective, low cost, innovative approach	Double sgRNA construction; need highly efficient sgRNA; special analysis
Target validation	Drug or anticancer reagent resistant cells	Lentiviral library from Addgene; or optional targets	Plasmid	Identify the target from resistant cells by sequencing	Effective	False-positives
Gene diagnose	Genome	Target sensitive genes	Lentivirus	Together with Cas13a or Cas12a to induce collateral effects	Sensitive, rapid, low cost	Certain template concentration