Table 1.
Crystallographic Data and Refinement Statistics.
| GFPuv form I | GFPuv form II | |
|---|---|---|
| Data collection | ||
| Space group | P21 | P21 |
| Unit cell dimension | ||
| a, b, c (Å) | 47.6, 51.0, 47.3 | 47.3, 51.2, 48.5 |
| β (°) | 98.5 | 100.8 |
| Resolution (Å) | 50.0–1.64 (1.68–1.64) | 50.0–1.28 (1.30–1.28) |
| Total reflections | 653331 | 367612 |
| Unique reflections | 26115 (1282) | 56911 (2566) |
| Completeness (%) | 95.7 (96.5) | 96.3 (87.4) |
| Rsym (%) | 5.9 (51.7) | 7.1 (24.8) |
| <I/σ(I)> | 27.4 (1.8) | 27.8 (7.2) |
| Refinement | ||
| R work | 0.188 (0.291) | 0.175 (0.195) |
| R free | 0.216 (0.322) | 0.195 (0.201) |
| Number of atoms | 2051 | 2292 |
| Protein | 1846 | 1886 |
| Ligand/ion | 31 | 38 |
| Water | 174 | 368 |
| B-factors | 32.9 | 16.8 |
| Protein | 32.1 | 14.5 |
| Ligand/ion | 31.9 | 15.9 |
| Water | 41.8 | 28.6 |
| R.m.s. deviation | ||
| Bond lengths (Å) | 0.007 | 0.006 |
| Bond angles (°) | 1.08 | 1.14 |
| Ramachandran plot | ||
| Favored (%) | 99.1 | 98.7 |
| Allowed (%) | 0.9 | 1.3 |
| Outliers (%) | 0 | 0 |