Table 4.
Ability of different fractions of polar compounds extracted from the strains isolated from Lomas Bayas soil to inhibit: 1, Acetobacter baumannii CL5973; 2, Escherichia coli ATCC 25922; 3, Klebsiella pneumoniae ATCC 700603; 4, Pseudomonas aeruginosa MB5919; 5,6 methicillin resistant/sensitive Staphilococcus aureus MB5393; 6, Staphilococcus aureus ATCC 23213; 7, Aspergilllus fumigatus ATCC 46645; 8, human hepatocellular carcinoma (HepG2) cells. Values under 50% are marked in bold.
| Strain ID | Fraction | Conc. [ug/mL] | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| % INH | SE | % INH | SE | % INH | SE | % INH | SE | % INH | SE | % INH | SE | % INH | SE | Conc. [mg/l] | Activity | SE | p-Value | |||
| LB4 | Water | 300 | 62.8 | 51.9 | −4.6 | 12.0 | −14.1 | 0.6 | 19.1 | 13.4 | 2.7 | 8.0 | 70.7 | 27.1 | −5.8 | 11.6 | 75 | −31.6 | 14.4 | 0.04 |
| LB4 | 25% methanol | 300 | −9.1 | 13.2 | −18.5 | 15.3 | −21.0 | 8.1 | −4.6 | 19.1 | −12.0 | 5.1 | −11.6 | 3.5 | −5.6 | 4.0 | 75 | 2.4 | 4.3 | 0.53 |
| LB4 | 50% methanol | 300 | −15.7 | 13.7 | −21.4 | 5.0 | −18.8 | 4.7 | −5.3 | 26.2 | −13.7 | 5.4 | −20.0 | 2.5 | −3.0 | 10.4 | 75 | 25.4 | 20.2 | 0.00 |
| LB4 | 75% methanol | 300 | 14.8 | 30.9 | −20.5 | 1.0 | −34.4 | 1.1 | 26.9 | 17.6 | −14.0 | 5.5 | −18.0 | 4.6 | 0.1 | 0.9 | 75 | 31.3 | 1.7 | 0.80 |
| LB4 | 100% methanol | 150 | 3.2 | — | −36.7 | 3.9 | −57.0 | 1.9 | 22.8 | — | −17.5 | 8.5 | −21.4 | 4.5 | −1.9 | 2.3 | 75 | −39.9 | 10.2 | 0.14 |
| LB4 | 100% methanol + 0.01% TFA | 150 | 58.0 | — | −28.5 | 6.5 | −53.9 | 2.3 | 21.5 | — | −13.6 | 10.6 | −11.8 | 1.7 | −0.1 | 2.5 | 75 | 27.6 | 2.7 | 0.70 |
| LB19 | Water | 300 | −8.6 | 17.5 | −4.3 | 6.7 | 3.2 | 30.9 | −4.8 | 20.6 | 3.0 | 20.3 | 50.0 | 33.2 | −1.4 | 5.3 | 75 | 10.8 | 13.8 | 0.05 |
| LB19 | 25% methanol | 300 | −17.5 | 7.5 | 5.1 | 5.8 | 1.8 | 12.4 | −6.2 | 21.4 | −15.2 | 3.3 | −10.5 | 1.4 | −7.3 | 10.3 | 75 | 10.9 | 5.3 | 0.45 |
| LB19 | 50% methanol | 300 | 62.0 | 34.7 | −9.8 | 0.2 | −20.1 | 0.0 | 23.0 | 24.4 | −13.9 | 6.1 | −2.2 | 4.0 | −3.0 | 7.1 | 75 | −24.3 | 13.0 | 0.06 |
| LB19 | 75% methanol | 300 | 67.2 | 21.9 | −18.4 | 1.6 | −36.3 | 2.7 | 31.6 | 32.7 | −12.6 | 6.4 | −18.5 | 1.4 | −4.5 | 3.7 | 75 | 18.6 | 12.8 | 0.07 |
| LB19 | 100% methanol | 300 | 83.4 | 24.4 | −29.4 | 3.5 | −46.1 | 5.7 | 39.4 | 42.6 | −17.7 | 5.8 | −15.9 | 11.1 | −2.4 | 8.9 | 75 | 17.1 | 7.5 | 0.28 |
| LB19 | 100% methanol + 0.01% TFA | 150 | 61.6 | — | −20.2 | 0.8 | −56.5 | 1.9 | 15.6 | — | −13.2 | 9.0 | −15.4 | 3.8 | −0.7 | 3.3 | 75 | −2.1 | 13.2 | 0.06 |
| LB32T | Water | 300 | 33.2 | 66.0 | −1.1 | 11.2 | −15.2 | 5.0 | −0.1 | 16.4 | 10.8 | 4.8 | 44.9 | 5.6 | −0.5 | 7.2 | 75 | −18.1 | 19.3 | 0.01 |
| LB32T | 25% methanol | 300 | −8.2 | 15.9 | 6.1 | 6.1 | −15.6 | 4.5 | −2.1 | 22.3 | −16.8 | 5.1 | −19.8 | 11.8 | −3.0 | 3.0 | 75 | 42.7 | 1.7 | 0.81 |
| LB32T | 50% methanol | 300 | 10.0 | 10.2 | −11.6 | 0.5 | −9.9 | 10.1 | 0.2 | 7.4 | −11.4 | 3.9 | −14.0 | 3.4 | 2.0 | 4.0 | 75 | 63.5 | 19.3 | 0.01 |
| LB32T | 75% methanol | 300 | −7.8 | 18.3 | −12.7 | 1.8 | −12.7 | 2.6 | −3.4 | 22.3 | −11.9 | 7.4 | −17.4 | 2.0 | −0.2 | 3.5 | 75 | 41.0 | 17.4 | 0.01 |
| LB32T | 100% methanol | 300 | 83.2 | 20.6 | −27.5 | 2.6 | −44.3 | 3.4 | 42.1 | 42.3 | −16.3 | 9.1 | −22.4 | 4.1 | −1.4 | 0.7 | 75 | 1.1 | 14.1 | 0.04 |
| LB32T | 100% methanol + 0.01% TFA | 300 | 60.6 | 17.7 | −29.6 | 0.2 | −58.6 | 0.8 | 33.2 | 25.3 | −18.2 | 6.0 | −20.0 | 3.2 | −6.3 | 1.9 | 75 | 31.2 | 6.2 | 0.37 |
| LB39T | Water | 300 | −8.7 | 17.3 | −9.4 | 3.1 | −26.1 | 3.0 | −2.9 | 23.2 | −12.5 | 8.5 | −12.5 | 2.9 | 1.3 | 3.2 | 75 | 14.3 | 8.9 | 0.20 |
| LB39T | 25% methanol | 300 | 3.8 | 41.7 | 1.7 | 0.3 | −16.6 | 3.8 | −12.4 | 16.7 | −16.7 | 5.2 | −18.1 | 0.5 | −3.4 | 5.3 | 75 | 35.0 | 0.9 | 0.89 |
| LB39T | 50% methanol | 300 | −6.5 | 15.7 | −11.2 | 4.3 | −21.9 | 2.8 | −3.8 | 17.2 | −13.1 | 6.8 | −20.9 | 0.4 | −1.7 | 2.6 | 75 | 25.5 | 20.8 | 0.00 |
| LB39T | 75% methanol | 300 | 57.5 | 54.9 | −27.5 | 0.2 | −30.9 | 1.9 | 10.5 | 15.8 | −12.8 | 4.2 | −11.9 | 3.1 | 1.9 | 6.5 | 75 | 26.0 | 10.4 | 0.13 |
| LB39T | 100% methanol | 300 | 75.2 | 26.1 | −26.7 | 3.3 | −37.8 | 1.6 | 32.5 | 38.7 | −17.1 | 7.8 | −18.2 | 0.0 | −1.8 | 4.1 | 75 | −5.1 | 0.9 | 0.90 |
| LB39T | 100% methanol + 0.01% TFA | 300 | 51.8 | 29.5 | −30.6 | 1.9 | −60.5 | 0.8 | 16.3 | 25.4 | −12.8 | 3.9 | −15.0 | 1.7 | −3.8 | 0.5 | 75 | 64.0 | 8.1 | 0.24 |
| LB41 | Water | 300 | −8.1 | 10.0 | −10.5 | 1.8 | −23.6 | 3.9 | −4.0 | 13.5 | −14.4 | 7.9 | −17.7 | 5.2 | 1.7 | 3.5 | 75 | −16.5 | 15.3 | 0.03 |
| LB41 | 25% methanol | 300 | −15.9 | 15.0 | −10.6 | 1.8 | −29.6 | 3.3 | −9.3 | 22.3 | −14.8 | 4.5 | −18.0 | 6.2 | −0.8 | 5.3 | 75 | 16.8 | 17.2 | 0.01 |
| LB41 | 50% methanol | 300 | 63.6 | 29.1 | −28.6 | 10.2 | −9.1 | 2.1 | 24.2 | 27.6 | −7.0 | 7.1 | −7.3 | 0.6 | −4.0 | 4.3 | 75 | 37.4 | 8.1 | 0.24 |
| LB41 | 75% methanol | 300 | 44.9 | 24.4 | −18.5 | 8.6 | −30.2 | 0.9 | 16.9 | 18.7 | −11.6 | 4.1 | −4.7 | 2.1 | −4.5 | 0.5 | 75 | 37.9 | 12.5 | 0.07 |
| LB41 | 100% methanol | 300 | 61.5 | 27.9 | −32.6 | 1.5 | −42.3 | 5.9 | 26.2 | 22.1 | −17.6 | 7.8 | −19.2 | 1.9 | −10.0 | 0.9 | 75 | −49.7 | 6.4 | 0.36 |
| LB41 | 100% methanol + 0.01% TFA | 300 | 30.1 | 62.3 | −14.4 | 2.1 | −6.7 | 1.2 | 0.9 | 18.6 | −10.7 | 8.4 | −17.3 | 3.7 | 1.4 | 2.7 | 75 | 47.1 | 0.3 | 0.97 |
| − | Negative Control | 150 | 9.0 | 10.9 | −8.0 | 1.1 | −2.0 | 5.8 | 17.4 | 1.8 | −8.0 | 1.9 | −8.0 | 1.5 | −5.0 | 2.8 | 500 | 4.8 | 14.6 | — |
| + | Positive Control (MMS) | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 4 | −99.9 | 0.2 | — |
% INH: percentage of inhibition; SE: Standard Error; TFA: trifluoroacetate; MMS: Methyl methane sulphonate.