Table 1.
Summary of the sequencing data and detected mutations
| Sample ID | Treatment | # Mutations | Callable sites (Mb) |
|---|---|---|---|
| A | Indoor | 0 | 126.4 |
| E | Indoor | 0 | 125.7 |
| I | Indoor | 0 | 126.0 |
| J | Indoor | 0 | 126.4 |
| N | Indoor | 0 | 125.9 |
| B | Outdoor-noUV | 0 | 126.1 |
| G | Outdoor-noUV | 1 | 126.3 |
| K | Outdoor-noUV | 0 | 124.2 |
| O | Outdoor-noUV | 0 | 125.9 |
| P | Outdoor-noUV | 0 | 126.4 |
| C | Outdoor-UV | 1 | 125.6 |
| D | Outdoor-UV | 1 | 126.3 |
| L | Outdoor-UV | 1 | 126.3 |
| M | Outdoor-UV | 0 | 126.3 |
| Q | Outdoor-UV | 0 | 126.0 |
Each row shows the sample information and number of verified mutations. Effective sites are estimated as the total number of sites with sufficient coverage for finding de novo variants using our pipeline. The mutation rate is calculated as μ = (number of mutations/sum of effective sites)/number of generations. The average mutation rates (95% confidence interval) for samples grown under indoor, outdoor-noUV and outdoor-UV conditions are: <7.92 × 10−11 (NA), 7.92 × 10−11 (2.07 × 10−11 to 3.98 × 10−10), and 2.38 × 10−10 (4.76 × 10−11 to 7.30 × 10−10), respectively. The 95% confidence intervals were calculated based on the assumption that the number of mutations is Poisson distributed