Table 1.

Tissue culture infectious dose (TCID₅₀/ml) of the recombinant pIY vaccine strain^a.

Recombinant pIY vaccine strain in different cell lines	TCID50/ml
pIY- infected RD cells	1.00 × 107
EV-A71 strain 41 in RD cells	5.60 × 102
pIY-scr-infected RD cells	4.47 × 102
pIY- infected SHSY-5Y cells	5.60 × 106
EV-A71 strain 41 in SHSY-5Y cells	0.30 × 10
pIY-scr-infected in SHSY-5Y cells	0.50 × 10
pIY-transfected NTERA-2 cells	1.85 × 102
EV-A71 strain 41 in NTERA-2 cells	5.61 × 10
pIY-scr-infected NTERA-2 cells	9.30 × 10

^aThe recombinant pIY vaccine strain carried 2 miRNA genes, as well as mutations in the 5′-NTR (∆11 bp) and G64R in the 3D polymerase.