Fig. 10.

Puzzle-fit mapping of histological BCC images to the fluorescent cancer signals. (a) The fresh tissue color image. (b) The registered fixed tissue image. (c) Autofluorescence image. (d) Probe fluorescence image. (e) H&E images and the “annotation lines” from trapezoidal projection method. (f) Fluorescence image fused with the “annotation lines.” The white outline of the fresh tissue border from (a) maps well to the registered fixed tissue image (b) and fluorescence images (c) and (d). Zoomed in view of center in H&E images from tissue blocks 8 and 9 are shown in (g) and (h), respectively. There is good correspondence between histological cancer annotation and cancer-probe fluorescence in tissue block 8, but not in tissue block 9.