Fig. 8.
The scoring mark point of an “annotation line” aids its localization in the bread-loaf block. (a) The bread-loaf block color image shows a thin scoring mark with a black ink smudge on the surface. Tissue sections for H&E are obtained from cuts into the tissue block with the orange frame outlining the starting surface. (b) The digitized H&E slide and corresponding “annotation line” that are obtained using the trapezoidal projection are shown. (c) The “annotation line” is moved along the tissue block image until the scoring mark corresponds to the black scoring mark. The line placement indicates the location of the acquired histological section. For almost all specimens, H&E slides are taken from the eastern (12 o’clock) edge. .