Figure 4.

Two-Component Systems of P. aeruginosa Regulate Swarming

(A–D) Perimeter versus normalized area plot for PA14 and 113 TCS mutants on (A) BM2 agar, (B) M8 agar, (C) M9 agar, and (D) PGM agar. Centroid for wild-type PA14 on respective media is shown. All mutants outside the centroids represent weak swarmers or non-swarmers.

(E) Venn diagram to show media-specific non-swarmer TCS mutants (also see Table S1).

(F) Representative images of swarm of six TCS mutants, rhlR and metR strains on BM2, M8, M9, and PGM media.

(G) Swimming phenotype of essential swarm regulators on LB-0.3% swim agar.

Mean values were compared by unpaired t test (p > 0.5, ns or not significant; ***p < 0.001). See also Figures S2–S4, and Table S1.