Figure 5.

Media-Dependent Role of TCS Genes in Swarming and Biofilm Formation

(A) Disk heatmap of swarm phenotype of 44 TCS mutants and rhlR. Heatmap is based on perimeter values in Table S1. Swarm phenotype: non-swarmer, perimeter less than 20% of PA14 swarm perimeter; weak swarmer, >20% but less than mean − SD of PA14 perimeter; hyper-swarmer, > mean + SD of PA14 swarm perimeter.

(B) Biofilm formation by 44 TCS mutants and rhlR in BM2, M8, M9, PGM, and M63 broths, measured by crystal violet stain (values in Table S3).

(C) Janus droplet representation of media-dependent biofilm and swarm phenotype. Left face shows swarming phenotype, whereas the right face reflects biofilm phenotype. Crystal violet staining for biofilm is represented as fraction of biofilm formation by PA14.

See also Figure S4, Tables S1–S3.