Figure 1. Phase-contrast micrographs of ARPE-19 and RPE cells from various donor ages.

ARPE-19 cells and RPE cells from the younger donors (9-20y) grew fast, the morphology was uniform and polygonal, had round nucleus, clear nucleolus and cytoplasm. The density of the cells reached above 90% within two to three days and then passaged. The cells were larger with increased aging, but all cultures showed robust pigmentation. Scale bar=30 µm.