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. 2019 Mar 12;10:299. doi: 10.3389/fpls.2019.00299

FIGURE 2.

FIGURE 2

The RT-qPCR analysis of the expression of the cloned gene Gh_D06G0281 (DTX/MATE) (A) Total RNA isolated from various tissue of cotton plant under normal conditions. (B) Total RNA extracted from salt-stressed cotton seedlings. (C) Total RNA extracted from drought-stressed cotton seedlings. (D) Total RNA extracted from cold-stressed cotton seedlings. (E) Polymerase chain reaction (PCR) analysis performed to check 1479 bp coding sequence (CDS) integration in transformed T1 generation, number 1–11 transgenic lines, 12 positive control (pWM101-Gh_D06G0281 (DTX/MATE) and 13 is the negative control (wild type). (F) The transcripts expression levels of the Gh_D06G0281 (DTX/MATE) of T2 transgenic lines analyzed through RT-qPCR, in three biological replicates.