FIGURE 4.

Alteration of leaf morphology, determination of oxidants and antioxidant enzymes concentration levels in Gh_D06G0281 (DTX/MATE) transgenic lines under salt, drought, and cold stress conditions. (A) Leaf size and pattern of each rosette leaf beginning from the oldest one of transgenic lines and wild types. The plants were grown for 8 days under 16 h light and 8 h dark photoperiod before photographs (B). Quantitative determination of malondialdehyde (MDA) concentration. (C) Quantitative determination of hydrogen peroxide (H₂O₂) concentration. (D) Quantitative determination of chlorophyll content. (E) Quantitative determination of POD concentration. (F) Quantitative determination of SOD concentration. (G) Quantitative determination of CAT in leaves of wild type and both DTX/MATE over-expressing (OE) lines (OE-2, OE-3, and OE-9) after 8-day post stress exposure. In (B–G), each experiment was repeated three times. Bar indicates standard error (SE). Different letters indicate significant differences between wild type and OE lines (ANOVA; p < 0.05). CK: normal conditions.