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. 2019 Mar 12;10:299. doi: 10.3389/fpls.2019.00299

FIGURE 5.

FIGURE 5

Overexpression of Gh_D06G0281 (DTX/MATE) causes hypersensitivity to ABA-elicited seed germination, root growth inhibition and fresh biomass accumulation in. transgenic plants and WT. (A) Seed germination rate of transgenic lines and wild type on ABA 0.5 MS with (0, 0.5, 1, and 2 μM) for 10 days. (B) Quantitative comparisons of germination rate of the transgenic and wild type on ABA 0.5 MS with (0, 0.5, 1, and 2 μM) after 10 days. (C) Root elongation comparisons on 0.5 MS with varied ABA concentrations 0, 0.5, 1, and 2 μM. The seedlings were scored and photographed after 6 days post germination. (D) Quantitative determination of root lengths. (E) Quantitative determination of fresh weight biomass of wild type and both DTX/MATE over-expressing (OE) lines (OE-2, OE-3, and OE-9) after 6 days post germination in different ABA concentrations. In (B,D,E), each experiment was repeated three times. Bar indicates standard error (SE). Different letters indicate significant differences between wild type and OE lines (ANOVA; p < 0.05). CK: normal conditions.