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. 2019 Mar 12;10:299. doi: 10.3389/fpls.2019.00299

FIGURE 6.

FIGURE 6

Overexpression of Gh_D06G0281 (DTX/MATE) effects on germination, root growth and fresh biomass accumulation in transgenic plants and wild type under osmotic stress. (A) Seed germination rate of transgenic lines and wild type on mannitol 0.5 MS with (0, 100, 200, and 300 mM) for 10 days. (B) Quantitative comparisons of germination rate of the transgenic and WT on mannitol 0.5 MS with (0, 100, 200, and 300 mM) for 10 days. (C) Root elongation comparisons on mannitol 0.5 MS with (0, 100, 200, and 300 mM). The seedlings were scored and photographed after 6 days post germination. (D) Quantitative determination of root lengths. (E) Quantitative determination of fresh weight biomass of wild type and both DTX/MATE over-expressing (OE) lines (OE-2, OE-3, and OE-9) after 6 days post germination in different mannitol concentrations. In (B,D,E), each experiment was repeated three times. Bar indicates standard error (SE). Different letters indicate significant differences between wild type and OE lines (ANOVA; p < 0.05). CK: normal conditions.