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. 2019 Mar 12;10:299. doi: 10.3389/fpls.2019.00299

FIGURE 8.

FIGURE 8

Overexpression of Gh_D06G0281 (DTX/MATE) enhances cold stress tolerance. (A) Seed germination rate of transgenic lines and wild type on 0.5 MS put at normal and low temperature (4°C) for 10 days. (B) Quantitative comparisons of germination rate of the transgenic and wild type on 0.5 MS put at normal and low temperature (4°C) for 10 days. (C) Root elongation comparisons on 0.5 MS put at normal and low temperature (4°C) for 6 days. The seedlings were scored and photographed after 6 days post germination. (D) Quantitative determination of root lengths. (E) Quantitative determination of fresh weight biomass of wild type and both DTX/MATE over-expressing (OE) lines (OE-2, OE-3, and OE-9) after 6 days post germination at normal and low temperature of 4°C. In (B,D,E), each experiment was repeated three times. Bar indicates standard error (SE). Different letters indicate significant differences between wild type and OE lines (ANOVA; p < 0.05). CK: normal conditions.