Table 3.
Differential expression of genes in cells overexpressing PSEN1 or PSEN1-P242LfsX11 in response to LPS in PMA-differentiated macrophages by PCR Array analyses
| Genes | Description | Fold change | Gene function |
|---|---|---|---|
| PSEN1 versus control overexpressed | |||
| TNF α a | Tumor necrosis factor α | 4.90 | Multifunctional proinflammatory cytokine, apoptosis |
| CCL13 | Chemokine (C-C motif) ligand 13 | 2.45 | Inducing chemotaxis, chronic inflammation |
| CXCL13 | Chemokine (C-X-C motif) ligand 13 | 2.11 | Lymphoid chemokine, chronic inflammation |
| CCL19 | Chemokine (C-C motif) ligand 19 | 2.04 | Lymphoid chemokine, chronic inflammation |
| PSEN1 versus control under-expressed | |||
| IL12A | Interleukin 12A | −3.34 | IL12 activate and link the innate and acquired immune responses |
| IL16 | Interleukin 16 | −3.18 | Binds and signals through CD4 receptor |
| LIF | Leukemia inhibitory factor | −3.14 | Mediates cell proliferation, differentiation and survival |
| IL12B | Interleukin 12B | −2.96 | Acts on T and natural killer cells |
| IL1B | Interleukin 1B | −2.64 | A subunit for IL12 and IL23; IL23 mediates late-stage inflammation |
| BMP2 | Bone morphogenetic protein 2 | −2.64 | Regulates the process of hair follicle regeneration |
| CSF2 | Colony stimulating factor 2 | −2.52 | Proliferation and differentiation of hematopoietic progenitor cell |
| CCL22 | Chemokine (C-C motif) ligand 22 | −2.16 | T-cell differentiation |
| IL23A | Interleukin 23A | −2.09 | IL23 mediates late-stage inflammation |
| IL10 | Interleukin 10 | −2.05 | Arrests and reverses the chronic inflammatory response |
| PSEN1-P242LfsX11 versus control overexpressed | |||
| CX3CL1 | Chemokine (C-X3-C motif) ligand 1 | 4.43 | The molecular control of leukocyte traffic at the endothelium |
| TNFSF11 | Tumor necrosis factor 11 | 2.55 | Regulates dendritic cell and osteoclast function; T-cell activation |
| IL11 | Interleukin 11 | 2.52 | The pleiotropic effects on hematopoietic cells |
| CCL17 | Chemokine (C-C motif) ligand 7 | 2.19 | Recruits selected subsets of leukocytes in inflammation |
| TNF α a | Tumor necrosis factor α | 2.00 | Multifunctional proinflammatory cytokine; apoptosis |
| PSEN1-P242LfsX11 versus control under-expressed | |||
| CSF2 | Colony stimulating factor 2 | −3.50 | Proliferation and differentiation of hematopoietic progenitor cell |
| IL5 | Interleukin 5 | −2.71 | Growth and differentiation factor for B cells and eosinophils |
| BMP2 | Bone morphogenetic protein 2 | −2.52 | Regulates the process of hair follicle regeneration |
| IL12B | Interleukin 12B | −2.10 | IL12 activate and link the innate and acquired immune responses |
| CXCL1 | Chemokine (C-X-C motif) ligand 1 | −2.09 | Directs and confines neutrophil influx to sites of injury |
| ADIPOQ | Adiponectin | −2.05 | Attenuate the inflammatory response |
| IFNG | Interferon γ | −2.05 | Critical for innate and adaptive immunity against bacterial infections |
| IFNA2 | Interferon α2 | −2.05 | Reduces inflammation |
| MSTN | Myostatin | −2.05 | Regulates embryonic development and maintains tissue homeostasis |
| THPO | Thrombopoietin | −2.05 | The megakaryocyte proliferation and lineage |
| XCL1 | Chemokine (C motif) ligand 1 | −2.05 | Induces the migration of cells expressing XCR1, including lymphocytes |
| NODAL | Nodal homolog | −2.05 | A long-range signaling molecule |
| IL4 | Interleukin 4 | −2.05 | B-cell stimulatory factor; T-cell and mast cell growth factor activities |
| IL21 | Interleukin 21 | −2.05 | Stimulates B-cell proliferation |
| IL3 | Interleukin 3 | −2.05 | The proliferation of hematopoietic cell |
| IL2 | Interleukin 2 | −2.05 | The T-cell growth factor maintaining the immune system |
| IL17A | Interleukin 17A | −2.05 | Stimulates cells to produce inflammatory mediators including IL1 |
| BMP7 | Bone morphogenetic protein 7 | −2.05 | Osteoblast differentiation in pluripotential and mesenchymal stem cells |
| CCL11 | Chemokine (C-C motif) ligand 11 | −2.05 | Recruitment of leukocytes to inflammatory lesions |
| LIF | Leukemia inhibitory factor | −2.02 | Mediates cell proliferation, differentiation and survival |
a TNF α exhibited overexpression, and LIF, IL12B, BMP2 and CSF2 exhibited under-expression in both PSEN1 and PSEN1-P242LfsX11 groups.