Table 1.
Primers used for real-time PCR
| Gene | Accession # | Primer sequence (AF; bp) | Gene | Accession # | Primer sequence (AF; bp) |
|---|---|---|---|---|---|
| β-actin | NM_001101.4 | F: 5-GCACAGAGC CTCGCCTTT-3 (230) | α-CAT | NM-001290307.2 | F: 5-AGCTAGCCG CAGAAATGACT-3 (212) |
| R: 5-GCCTCGTCG CCCACATAG-3 | R: 5-TTCAACAGA TGCAGCCAAAAC-3 | ||||
| NDRG2 | NM_001354558.1 | F: 5-CCTCACCTC TTCCATTCC-3 (188) | β-CAT | NM-001330729.1 | F: 5-ACGGAGGAA GGTCTGAGGAG-3 (211) |
| R: 5-TATCACCTC CACGCTCAA-3 | R: 5-AGCCGCTTT TCTGTCTGGTT-3 | ||||
| E-CAD | NM_001317185.1 | F: 5-AGGCAAGGT TTTCTACAGCAT C3 (154) | Snail | NM_005985.3 | F: 5-GGAAGCCTAA CTACAGCGAGC-3 (151) |
| R: 5-TGACACAG CGTGAGAGAAG AGAG-3 | R: 5-AGGACAGAG TCCCAGATGAGC-3 | ||||
| TGF-β | NM_000660.6 | F: 5-AGTGGTTGA GCCGTGGAGG-3 (169) | Slug | NM-003068.4 | F: 5-AAGGACACA TAGAACTCACAC G-3 (195) |
| R: 5GCCATGAGA AGCAGGAAAG-3 | R: 5-CACAGCAGC CAGATTCCTCA-3 | ||||
| VEGF | NM_001171622.1 | F: 5-GGGCAGAAT CATCACGAAGT-3 (238) | TGF-α | NM-001099691.2 | F: 5-CTGGGTATT GTGTTGGCTGC-3 (171) |
| R: 5-GGTGAGGTT TGATCCGCAT-3 | R: 5-TGCTGGCTT G TCCT CCTGC-3 |
NM: Accession number; AF: Size of amplified fragment; bp: Base pair