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. Author manuscript; available in PMC: 2019 Mar 19.
Published in final edited form as: Methods Enzymol. 2018 Dec 28;616:265–288. doi: 10.1016/bs.mie.2018.10.029

Fig. 2.

Fig. 2

Schematics of the library-based method for identification of AceCas9 PAM. (A) Schematic of pUC19 library containing randomized PAM region and a BamHI cleavage site, the products generated by AceCas9 and BamHI cleavage, and the sequenced consensus at the PAM. (B) Agarose gel analysis of AceCas9 cleavage of the pUC19 vector with the identified PAM sequence (5′-NNNCC-3′) and its variants.