Fig. 3.
Microbial modulation of locomotor activity and anxiety-related behavior. (A,B) At 6 dpf, germ-free (GF) zebrafish larvae display increased locomotor activity compared to larvae raised in a conventional environment (CV and CR). (C,D) GF larvae exhibit less thigmotactic behavior than CV and CR larvae as indicated by number of entries into the center zone (C) and time spent in the center zone (D). Data shown are represented by box and whisker plots showing individual data points (n = 36–87 larvae/group). Bars denote p values ≤0.05 (one way ANOVA).