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. 2019 Mar 12;10:224. doi: 10.3389/fphys.2019.00224

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Copyright © 2019 Jauhiainen, Laakkonen, Ketola, Toivanen, Nieminen, Ninchoji, Levonen, Kaikkonen and Ylä-Herttuala.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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VEGF-A-stimulated upregulation of FLRT3 was detected also in microvascular ECs. (A) Single-cell transcriptomic data from mouse lung tissue show notably higher expression level of FLRT3 mRNA in capillary ECs than other EC clusters. (B) Comparative qPCR analysis for basal expression level of FLRT3 mRNA in HUVECs and microvascular ECs (HDMEC). (C) Significant upregulation of FLRT3 mRNA was detected in HDMECs 1 and 3 h after VEGF-A-stimulation. (B,C) The data presented as mean ± SEM are representative of repeated independent experiments done in triplicates. ^∗p < 0.05; ^∗∗∗p < 0.001.