Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 12;10:224. doi: 10.3389/fphys.2019.00224

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Jauhiainen, Laakkonen, Ketola, Toivanen, Nieminen, Ninchoji, Levonen, Kaikkonen and Ylä-Herttuala.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Efficiency of siRNA oligonucleotides targeting to FLRT3. (A) Three specific siRNA oligonucleotides targeting FLRT3 were tested. (B) FLRT3 siRNA #1 was chosen for further studies and it was able to cause a strong inhibition in the expression of FLRT3 mRNA in non-stimulated HUVECs as well as in the cells stimulated with VEGF-A (50 ng/ml) at both of the tested concentrations but did not affect VEGFR-1 (C), VEGFR-2 (D), or VEGFR-3 (E) expression in non-stimulated cells. The data presented as mean ± SEM are representative of repeated independent experiments done in 3–5 replicates. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; #p < 0.01 as compared to corresponding cells transfected with mock or control siRNA; and ns, non-significant.