Table 2.
List of proteins with significantly different levels in trophozoites of all wild-type (WT) and all nitro drug resistant (RES) strains. The resistant strains were grown in the presence of 50 μM metronidazole (MET) or nitazoxanide (NTZ) as described in Materials and methods. Vsp, variant surface protein; nd, not detected; su, subunit. For each strain, three biological replicates have been tested (with three technical replicates per biological replicate).
| Differential expression | Nitro compound | Annotation | Giardia DB |
|---|---|---|---|
| Down-regulated in RES vs. WT | |||
| NTZ | |||
| GTP binding ADP ribosylation factor domain-1 protein | 8140 | ||
| Intramembrane protease (minor histocompatibility antigen H13) | 8429 | ||
| Hypothetical (membrane spanning) | 114623 | ||
| MET | |||
| Hypothetical (membrane bound) | 3158 | ||
| nuclear LIM Interacting factor 1 | 4063 | ||
| High cystein membrane protein | 9620 | ||
| Dynein intermediate chain | 10254 | ||
| Hypothetical (RNA binding) | 14117 | ||
| Hypothetical | 16793 | ||
| ATP-dependent RNA helicase | 16887 | ||
| Vsp-3 | 137740 | ||
| Vsp-8 | 137618 | ||
| Vsp-77 | 137617 | ||
| Vsp-88 | 101074 | ||
| Vsp-160 | 137612 | ||
| Both | Hypothetical (transmembrane) | 34701 | |
| Up-regulated in RES vs. WT | |||
| NTZ | |||
| Phosphatase 1 regulatory subunit | 11885 | ||
| Hypothetical (serine rich adhesin) | 94542 | ||
| MET | |||
| Hypothetical (nuclear protein) | 3021 | ||
| High cystein membrane protein group 1 | 16318 | ||
| CXC-rich protein | 17476 | ||