Table 3.

Antigenic complexity in nitro drug resistant G. lamblia lines (C4, 10621D10, 713M3; grown on NTZ or MET) is not altered as compared to their corresponding wild-types (WBC6, 106, 713). The strains were grown (the resistant strains in the presence of 50 μM MET or NTZ), harvested and subjected to MS shotgun analysis as described in Materials and methods. For each strain, three biological replicates have been tested (with three technical replicates per biological replicate). Total numbers of proteins either annotated as variant surface proteins, high cysteine surface proteins or CXC rich surface proteins are given. The proteins were classed according to their expression levels determined via the LFQ algorithm. The complete dataset concerning this group of proteins is listed in Supplementary Table S2.

LFQ (x106)	WBC6			106			713
	WT	NTZ	MET	WT	NTZ	MET	WT	NTZ	MET
>104 - 103	1	1	1	0	0	0	2	3	0
103–102	11	12	8	1	0	0	6	4	6
102–101	31	40	28	10	17	9	27	25	21
101–100	19	25	25	16	16	17	25	24	26
total	62	78	62	27	33	26	60	56	53