Table 4.

Overview of proteins involved in reduction (and thus activation) of nitro compounds and the scavenging of radicals or other toxic intermediates as a consequence of this reduction in trophozoites of wild-type (WT) and nitro drug resistant (RES) strains, the latter grown in the presence of 50 μM metronidazole (MET) or nitazoxanide (NTZ). Cells were harvested and subjected to MS shotgun analysis as described in Materials and Methods. For each strain, three biological replicates have been tested (with three technical replicates per biological replicate). For all proteins, mean values ± standard errors for LFQ intensities (x10⁶) in three biological replicates are given (nd, below detection limit). DB, number in GiardiaDB; Fd, ferredoxin; Hb, hemoglobin; FP, flavoprotein; LT, lateral transfer; NO, NAD(P)H oxidase; NR, nitroreductase; PFOR, pyruvate-ferredoxin oxidoreductase. *, two-sided t-test comparing the resistant strains to their respective wild-types, p < 0.001.

Annotation	DB	WBC6			106			713
		WT	MET	NTZ	WT	MET	NTZ	WT	MET	NTZ
Fd-NR1 ("NR2")	6175	nd	nd	nd	nd	nd	nd	nd	nd	nd
Fd-NR2 ("NR1")	22677	137 ± 8	92 ± 5	58 ± 1*	7 ± 0	5 ± 0	5 ± 0	42 ± 4	45 ± 3	36 ± 1
NR family	15307	nd	nd	nd	nd	nd	nd	nd	nd	nd
PFOR 1	17063	4602 ± 73	3173 ± 100	4561 ± 132	713 ± 24	240 ± 23*	292 ± 6*	4191 ± 199	3735 ± 40	5146 ± 50
PFOR 2	114609	7510 ± 193	4186 ± 9	7846 ± 110	987 ± 23	501 ± 65*	812 ± 8	3623 ± 154	4159 ± 87	5071 ± 96
TrxR	9827	393 ± 6	621 ± 20*	395 ± 25	122 ± 31	163 ± 30	115 ± 13	533 ± 62	1001 ± 12*	558 ± 111
Flavo-Hb	15009	nd	nd	nd	nd	nd	nd	nd	2 ± 0*	2 ± 0*
A-type FP	10358	927 ± 35	606 ± 14*	1019 ± 35	178 ± 6	178 ± 22	254 ± 5	933 ± 21	2076 ± 49*	2070 ± 132*
NOLT	33769	3631 ± 121	2646 ± 81	4463 ± 277	577 ± 33	514 ± 15	641 ± 27	2938 ± 94	2594 ± 113	2827 ± 54
NO	9719	451 ± 22	376 ± 17	428 ± 14	90 ± 4	85 ± 5	83 ± 1	390 ± 3	470 ± 6	425 ± 3