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Male sex specific markers of size ∼290 and ∼230 bp amplified by SRAP primer combination 'Em-6/Me-4' in different T. dioica plants growing in Kanpur, North Delhi, Meerut, Baghpat, Samastipur, Saran, Muzaffarpur and Gopalganj regions. The arrows indicate male specific bands of ∼290 and ∼230 bp sizes present in only male bulk and male individuals. M: 3 kb ladder, PF: Pooled female, PM: Pooled male.
