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. 2019 Feb 20;19(4):3132–3138. doi: 10.3892/mmr.2019.9973

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Roles of PTEN in the pathogenesis of glaucoma. (A) Expression of PTEN in HTMCs under oxidative stress by western blot method; (B) Effect of overexpression and knockdown of miR-17-5p on the mRNA expression of PTEN in HTMCs by RT-qPCR method; (C) Effect of overexpression and knockdown of miR-17-5p on the protein expression of PTEN in HTMCs using ICC method (scale bar, 100 µm). **P<0.01 vs. NC. NC, un-transfected cells; Inhibitor, miR-17-5p inhibitor transfected cells; Mimics, miR-17-5p mimics transfected cells. PTEN, Phosphatase and tensin homolog; HTMCs, human trabecular meshwork cells; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.